KOREAN  ENGLISH

시약 및 원료

크마토그래피

실험실기초장비

분석장비

연구소모품

회사소개

    주요취급품목

 

    Detectors

    Chromatography Gels

PolySULFOETHYL A™

- for Cation-Exchange of Peptides -

    This strong cation-exchange (SCX) material was developed specifically for HPLC of peptides. At pH 2.7-3.0, peptides lose their (-) charges and have net (+) charge. Thus, PolySULFOETHYL A™ is a general-purpose alternative to reversed-phase (RPC), fractionating peptides by differences in charge rather than polarity. Compared to other SCX materials based on sulfopropyl- (SP-) groups, PolySULFOETHYL A™ is unusually hydrophilic. This minimizes hydrophobic interactions with peptides, with high recovery and less peak tailing. Capacity is also high, permitting better retention and fractionation of the weakly basic peptides from tryptic digests. The capacity of an ion-exchange material like this is 4x that of a comparable RPC column. Therefore, ion-exchange should be the initial step of a multi-step purification.  Use PolySULFOETHYL A™ for:

    1) Multidimensional HPLC of peptide mixtures, such as tryptic digests in proteomics analyses (including iTRAQ® and ICAT®* reaction products).

    2) Isolation of peptides from natural products.

    3) QC and purification of synthetic peptides.

    4) Selective isolation of disulfide-linked peptides, phosphopeptides and C-terminal fragments from tryptic digests.

    5) Mapping of peptide digests (tryptic, V8, CNBr, etc.).

    Proteins can be run on     PolySULFOETHYL A™ columns too; at pH 3, retention is all but guaranteed. An example is the analysis of     Lung Surfactant Protein on a PolySULFOETHYL A column operated in the Hydrophilic Interaction (HILIC) mode.

    Standard material for peptide applications is 300-Å, in either 3- or 5-µm. The 200-Å material has about 25% greater capacity and is preferred for phosphopeptide isolation and fractionation of iTRAQ® reaction mixtures. For proteins, use 1000-Å material or the 3-µm, 1500-Å material.

    PolySULFOETHYL A™ Instruction Sheet

    MSDS Instruction Sheet 

    PolySULFOETHYL A™ is a trademark of PolyLC Inc.

    *ICAT® and iTRAQ® are trademarks of Applied Biosystems Inc., a branch of Applera Corp.

    주소

서울특별시 송파구 충미로 5 송파한화오벨리스크 C동 415호

    연락처

전화번호 : 02-3012-9003           팩스번호 : 02-3012-9010

    E-mail

intertech9@naver.com

    사업자등록번호

215-87-83507                                 대표이사 : 이홍근